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Undergraduate Research Posters 2008
Show moreAntibiotic resistance often originates from a bacteria's ability to produce enzymes known as Beta-lactamases are known to exist and they are divided into four different classes (class A, B, C, D) depending on their structure. The SHV family of enzymes presented here belong to class A of serine Beta-lactamase.
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Show moreThe pedal skeletons of 2,614 individuals from the Hamann and Todd osteological collection at the Cleveland Museum of Natural History are assessed for the human skeletalanomaly medial cuneiform bipartum. Fourteen individuals, a total of nineteen medial cuneiforms, are positive for the condition.
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Show moreActivating mutations in SHP-2 tyrosine phosphatase implicated in cell signaling processes have been identified in various childhood leukemias and sporadic solid tumors. However, the molecular mechanisms by which the activating mutations in SHP-2 induce malignancies are not fully understood. In this study, we investigated potential involvement of SHP-2 in chromosome segregation during mitosis, dysregulation of which leads to genome instability and malignancies. We determined localization of SHP-2 in the kinetochore, a crucial protein complex structure connecting microtubules and chromosomes. We found that SHP-2 localized at the kinetochore. Our findings suggest that SHP-2 may be involved in chromosome segregation and can help to answer how SHP-2 mutations can induce genetic instability and thereby malignancies.
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Show moreThe long term project will measurephosphorylated signaling molecules in hematopoieticstem cells and committed progenitorsin normal and acute myeloid leukemia(AML) samples. It will involve the evaluationof the phosphorylation state of the intracellular signaling molecules, ERK and the ribosomalprotein S6, in bone marrow cells from donors.In this short term project, the chronic myelogenousleukemia (CML) cell line, K562 (a type ofHematopoietic Stem Cells (Fig. 1)), was used in place of bone marrow cells to develop analyticalmethod.
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Show moreCited2 (CBP/p300-interacting transactivator with glutamic acid (E)/aspartic acid (D) rich C-terminaldomain) is a member of the transcription factor modulators family. Cited2 is essential for normal development in mice. Mice with a total knockout (KO) of this gene died within the first 2-3 weeks of development and presented malformations in liver, eye lens, and heart. In the liver Cited2 is known to interact with HNF4-alpha to mediate functions essential for liver development, differentiation and functions. Our main focus is to identify the role of Cited2 in the adult liver by performing in vivo and in vitro experiments. In our in vivo experiment, Cited2 liver specific KO mice were generated followed by analyzing their blood biochemistry and glucose tolerance. Liver specific KO mice and wild type mice did not present differences in their blood biochemistry and their body weight. In contrast, a difference in glucose tolerance was observed between KO and wild type mice. In the in vitro experiment, the effect of Cited2 expression on cell proliferation was studied in a human liver cell line, Sk-Hep1. These cells were treated with butyrate (a HDAC inhibitor) and viability was measured by the MTT assay and Cited2 mRNA expression was analyzed using Real Time PCR. The data showed that butyrate (HDAC inhibitor) affects the expression of Cited2 and cell proliferation. Taken together, these results suggest Cited2 may play a role in liver functions in adult mice. More detailed experiments will have to be pursued in order to find specific pathways involved.
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